FastQCFastQC Report
Wed 25 May 2016
SRR2074883_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074883_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1507895
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCG3900.040.84467321
TAGCGGA4700.038.4879734
ATTTACG4100.037.53522554
AGCGGAT5050.036.8897535
GGTAGCG5050.034.21658332
CGTATGC2750.032.39882741
TATGCCG2800.031.82027243
GCGGATG6150.031.16960536
GTAGCGG5600.030.85602233
TTGGCGT5200.030.63350522
TCGTATG2850.030.31469340
GAGGCGC5900.030.20329713
AAGGTAG5750.029.58153530
GCGTGAA5700.028.89369225
CGGATGA6500.028.66050137
ATGCCGT2900.027.93002144
TGAGGCG6800.027.39788212
GAACATG14750.026.7297741
GAAGGTA6450.026.37113829
GGCGCCA7100.026.23935515
Produced by FastQC (version 0.11.5)