FastQCFastQC Report
Wed 25 May 2016
SRR2074882_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074882_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences576527
Sequences flagged as poor quality0
Sequence length60
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG850.041.29365539
CGCCCTA755.456968E-1239.59955237
GGGTTAG1450.037.240961
CGTGGTA950.036.94695342
GCGTCGT950.036.94695338
AGCGTCG1050.035.99959637
GAACATG7850.033.3626821
GTAGCGT1200.031.49964535
AACATGG8300.030.577972
TCGTGGT1150.030.52139741
TAGCGTC1250.030.2396636
GAATCAA4950.029.9996641
AACGCCC901.7007551E-929.9996635
ACCGACT1350.028.00211513
CCGATCG400.0086036927.00203914
CGCGGGT400.00860733126.99969929
TGCGTTA400.00860733126.99969948
ACGCCGA706.574859E-626.99969742
AATCAAC5500.026.9996952
GGGTAGT1204.5474735E-1126.99969524
Produced by FastQC (version 0.11.5)