FastQCFastQC Report
Wed 25 May 2016
SRR2074881_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074881_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1425570
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT3050.042.49031435
TAGCGTC3150.041.9985336
GCGTCGT3100.041.80498538
CGTGGTA3000.040.49857742
AGTAGCG3400.039.7044934
AGCGTCG3450.038.3464837
ATTTACG2300.037.5652254
ATTGGCG2350.035.6157721
CGTCGTG3550.034.2241539
AGCGGAT3050.031.86773735
TCGTGGT3800.031.26206241
CGCGAAT350.00448589230.85606243
TAGCGGA2950.030.20233234
GTAGCGG2950.030.20233233
GGGTAGT4300.028.8827124
GGTAGCG3150.028.28472332
TCGTGCG806.0817183E-727.049
TAACTCG400.00861103827.049
GTGTAAG4700.026.9990512
GGTATTC3450.026.60869645
Produced by FastQC (version 0.11.5)