FastQCFastQC Report
Wed 25 May 2016
SRR2074880_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074880_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316607
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCCATAATTTACG15170.11522041125407961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGAT5100.045.5286135
TAGCGGA5150.044.56232534
ATTGGCG5300.042.28227221
ATTTACG5050.041.1691654
GGTAGCG5950.039.02452532
TTGGCGT6200.036.14452722
GTAGCGG6500.035.7224533
AAGGTAG6900.033.65158530
GCGGATG7050.033.3185636
GAGGCGC7200.032.62566813
GCGTGAA6650.032.07462325
AGGTAGC7250.032.02702331
GCGCCAT7300.031.80886816
TATTGAG7300.031.4389979
GGCGCCA7650.031.06063815
TATGCCG2000.031.04945443
CGGATGA7450.030.80482937
AGCCATA6200.030.04900247
TGAGGCG8750.029.93204112
CGACAAT1300.029.07751717
Produced by FastQC (version 0.11.5)