FastQCFastQC Report
Wed 25 May 2016
SRR2074878_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074878_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622613
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG150.00621838353.99855434
GGCGCGA150.00621838353.99855410
CGTCGTG1400.040.49891739
TAGCGTC1500.037.7989936
GTAGCGT1500.037.7989935
GCGTCGT1450.037.24038338
CGTGGTA1550.036.57966642
AGTAGCG1550.036.57966634
TATGCCG901.8189894E-1235.99903543
GTCGTGG1650.034.36271740
ATGCCGT951.8189894E-1234.1043544
GACGGCA1900.032.68333426
AGCGTCG1800.031.49915737
CCGTCTT958.913048E-1131.26232147
ACCCGAG350.004478505330.8637522
AGACGGC2100.030.85631825
CGGCATA1800.029.99919728
GGCATAG1800.029.99919729
AAGAGCG2550.029.6462657
ACGGCAT2100.028.28495827
Produced by FastQC (version 0.11.5)