FastQCFastQC Report
Wed 25 May 2016
SRR2074877_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074877_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444648
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC7860.17676903977978084No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAC150.00621691953.99921431
AGTAGCG1550.047.03157434
GCGTCGT1450.046.5510538
TAGCGTC1550.045.2896636
GTAGCGT1600.043.8743635
CGTGGTA1600.042.18688642
CGTCGTG1600.042.18688639
AGCGTCG1750.038.57086637
TAGCGGA850.038.11709234
TCGTGGT1950.037.3840741
AGCGGAT801.2732926E-1137.1244635
GCGAACC300.002105872835.9994774
TAACGCA451.0016975E-535.9994774
GTCGTGG1950.035.99947440
GTGTAAG2100.035.99947412
GGTAGTC1950.035.99947425
GGTATTC1450.035.37879645
GGGTAGT2000.035.09948724
GTAGTCT2000.035.09948726
CTGGGTA2200.033.13588322
Produced by FastQC (version 0.11.5)