FastQCFastQC Report
Wed 25 May 2016
SRR2074876_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074876_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993658
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC53330.2674982369092392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG11650.045.42358439
GCGTCGT12050.044.1398138
TAGCGTC12050.043.9157536
GTAGCGT11950.043.8313735
CGTGGTA11750.043.65831442
AGCGTCG12700.042.30586637
GGTATTC8950.041.63113445
AGTAGCG12800.041.55349334
GTCGTGG13350.039.8415540
TCGTGGT13400.038.68545541
GGGTAGT13850.038.40419424
GGTAGTC14150.037.3991625
GAGTAGC15250.035.58585733
GTATTCC10400.035.3075646
TATGCCG3950.034.85975343
CTGGGTA14800.034.84446722
GTGTAAG14200.034.7973912
GGTGTAA14600.034.0298311
AAGCATC15800.034.00790816
TAAGCAT15400.033.83923315
Produced by FastQC (version 0.11.5)