FastQCFastQC Report
Wed 25 May 2016
SRR2074875_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074875_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891295
Sequences flagged as poor quality0
Sequence length60
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCCATAATTTACG10430.11702073948580435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCG2700.045.9983321
TTGGCGT3000.040.49852422
AGCGGAT3250.039.0447335
ATTTACG3000.038.6985954
GGTAGCG3150.038.57002632
TAGCGGA3300.036.8168434
TATGCCG704.8858055E-934.71302443
GGCGCCA4100.033.5841515
GAGGCGC4550.030.8560213
GCGCCAT4100.030.29158416
GCGGATG4400.030.0670936
AGCCATA4100.029.6330747
GCCATAA4050.029.33226648
TGAGGCG4700.029.29680812
AGGTAGC4350.029.17135231
CGGATGA4650.029.031237
CATTGGC4200.028.9275220
GTAGCGG4200.028.9275233
GAAGGTA4650.027.86995329
ATTGAGG5050.027.27092410
Produced by FastQC (version 0.11.5)