FastQCFastQC Report
Wed 25 May 2016
SRR2074874_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074874_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109915
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC18850.1698328250361514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT4500.047.39972335
TAGCGTC4600.046.36929336
CGTCGTG4600.046.36929339
CGTGGTA4700.045.9571842
AGCGTCG4900.044.6323937
AGTAGCG5100.043.4115134
GCGTCGT5050.042.77202638
TCGTGGT5100.042.35269541
GTCGTGG5500.039.27249540
GGGTAGT6100.037.1801124
GGTATTC4250.036.21155545
CTGGGTA6900.034.8274522
GGTAGTC6850.033.8976125
GAGTAGC6700.033.44756733
TGGTGTA6550.033.3891210
AAGCATC6900.033.26217316
GTGGTGT6800.032.955699
ATCTGGG7300.032.5492220
TAAGCAT6900.032.4780715
TATGCCG1850.032.1079243
Produced by FastQC (version 0.11.5)