FastQCFastQC Report
Wed 25 May 2016
SRR2074873_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074873_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1680337
Sequences flagged as poor quality0
Sequence length60
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG3150.032.5705340
TATGCCG3150.031.71341143
ATTTACG1700.030.17653754
CGTATGC3250.029.0761241
AGCGGAT2250.027.59924135
ATTGGCG2200.026.99925621
TAGCGGA2250.026.39927334
CTCGTAT4050.025.99928539
TCTCGTA4000.024.97431238
ATGCCGT3750.024.48005344
GGTAGCG2550.024.3522732
TTGGCGT2400.023.6243522
CCGTCTT3500.023.1429147
ACGAGAC5850.023.07628822
ATCTCGT4400.022.7039237
GCCGTCT3750.022.3200546
GGTATCA47200.022.1976051
CCTAGGT5600.021.69583128
CTAGGTT5800.021.41320429
TGCCGTC4200.021.21433345
Produced by FastQC (version 0.11.5)