FastQCFastQC Report
Wed 25 May 2016
SRR2074872_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074872_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447606
Sequences flagged as poor quality0
Sequence length60
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6640.14834474962355285No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5290.11818429601033052No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5190.11595018833527701No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5170.11550336680026631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG1900.039.7894741
CGTGGTA950.036.9473742
AGCGTCG950.036.9473737
GCGTCGT950.036.9473738
GTAGCGT1100.036.81818435
CACGTAT300.002105737636.018
TACCGTA300.002105737636.07
TTAGAGC2000.035.1000024
TAGCGTC1150.032.86956436
CGTCGTG1100.031.90909239
GTTAGAG2150.031.395353
ACGTATG350.004481868430.85714319
CCGAACA350.004481868430.85714333
AGTAGCG1400.030.85714334
GTGGTTA2400.030.3751
TCGTGGT1201.8189894E-1229.2541
TGGTTAA2450.028.653062
GGTTAAG4100.027.6585353
ATCGTTC400.00860483927.033
GGTATTC1104.8748916E-1027.045
Produced by FastQC (version 0.11.5)