FastQCFastQC Report
Wed 25 May 2016
SRR2074871_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074871_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1399532
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT2500.042.11944238
CGTCGTG2850.037.89423439
TCGTATG3350.037.88009640
TAGCGTC2800.036.64237236
TATGCCG3050.036.294643
CTCGTAT3500.036.25666439
AGCGTCG2950.035.69444337
CGTGGTA3250.034.89184642
GTAGCGT3050.033.63889735
TCTCGTA3550.031.9432438
TCGTGGT3650.031.06808341
AGTAGCG3650.030.32836734
CCGTCTT3150.029.99960347
GCCGTCT3200.029.53085746
CGTATGC3950.029.39201741
TAGCGGA2050.028.97522734
GTCGTGG4200.028.92818840
ATTTACG1550.027.870654
ATGCCGT3800.027.71015744
TGGGTAG4250.026.68223
Produced by FastQC (version 0.11.5)