FastQCFastQC Report
Wed 25 May 2016
SRR2074870_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074870_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2188771
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC32590.14889634411274638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT9700.043.1437135
TAGCGTC9900.042.8175736
CGTCGTG9750.042.091739
CGTGGTA9950.041.2456442
GCGTCGT10050.040.83523638
AGCGTCG10800.039.2494437
AGTAGCG11150.039.22813834
TCGTGGT10800.037.9994641
GGGTAGT11500.036.15624
GTCGTGG12000.035.5494940
GGTATTC9950.033.91988445
GAGTAGC13450.033.3229433
GTGTAAG13200.032.31845512
TAGCGAT2600.032.19184532
GGTAGTC13300.031.26270925
TGGGTAG13600.031.1686723
TGAGTAG14050.031.13122632
CTGGGTA13650.031.05449922
TAAGCAT14100.031.02295515
GGTGTAA14100.030.63855711
Produced by FastQC (version 0.11.5)