FastQCFastQC Report
Wed 25 May 2016
SRR2074869_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074869_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1554860
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC22690.14592953706443024No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15830.10180980924327591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT5750.040.85159335
CGTCGTG5700.040.73626339
CGTGGTA5950.039.93220542
TAGCGTC5950.039.93220536
AGCGTCG6400.038.39007637
GCGTCGT6050.038.37961638
GTCGTGG6300.037.7137540
AGTAGCG6400.036.70260234
GGTATTC5600.034.71490545
TCGTGGT7000.034.7137941
GGGTAGT7400.031.01307124
GTATTCC6650.030.04564946
GGTAGTC7600.029.8416825
GTGTAAG8350.029.42472812
CTGGGTA8000.028.6870922
ACCGTAG1052.8558134E-1028.28530913
GAGTAGC8600.028.2554133
TGGTGTA8350.027.80798510
TGGGTAG8500.027.634923
AAGCATC9000.027.59960616
Produced by FastQC (version 0.11.5)