FastQCFastQC Report
Wed 25 May 2016
SRR2074868_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074868_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060164
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC16960.1599752491124015No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11570.10913405850415596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTA4000.042.52585642
GTAGCGT4200.041.78458435
CGTCGTG4200.041.14368439
TAGCGTC4350.040.9644136
AGCGTCG4400.040.49890537
AGTAGCG4500.040.1989134
GCGTCGT4400.039.88716538
GTATTCC3900.035.30673646
GGTATTC3900.035.30673645
TCGTGGT4900.035.26601441
GTCGTGG5150.033.02979340
TACGTTC502.0629352E-532.40065412
CTGGGTA5600.031.33991622
GGGTAGT6000.031.05208824
GTGTAAG5950.030.40397512
GTAAGCA6100.030.09896514
TAAGCAT6000.029.70059415
GTTTCAT6100.029.6549342
ATCTGGG6050.029.45513720
TATGCCG1400.028.92915343
Produced by FastQC (version 0.11.5)