FastQCFastQC Report
Wed 25 May 2016
SRR2074867_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074867_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412619
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCG2750.038.2909121
TAGCGGA3150.037.71428734
ATTTACG2950.037.52542554
GCGTCGT3200.037.12538
GGTAGCG3550.035.7464832
AGCGTCG3600.034.537
GTAGCGT3450.034.43478435
CGTCGTG3500.033.9428639
CGTGGTA3450.033.65217242
AGTAGCG3800.033.39473734
TAGCGTC3550.032.70422736
AGCGGAT3800.031.97368435
TATCACG1251.8189894E-1228.0799982
GTAGCGG4250.027.9529433
GGGTAGT4350.027.93103424
GAGGCGC4600.027.58695613
TTGGCGT3750.027.3622
ACGTTAG3850.027.35064927
TCGTGGT3950.027.34177241
GTCGTGG4150.027.32530240
Produced by FastQC (version 0.11.5)