FastQCFastQC Report
Wed 25 May 2016
SRR2074864_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074864_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1566548
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC24550.15671399791133117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG5100.044.46935339
GCGTCGT5250.044.2273538
GTAGCGT5500.043.198835
CGTGGTA5250.042.68453242
TAGCGTC5700.041.68305236
AGCGTCG5750.041.3205937
AGTAGCG6100.040.2775734
GGTCGAC652.3683242E-937.3859632
GGGTAGT6800.034.5442624
TCGTGGT6750.033.99905841
GGTATTC5850.033.22984745
GTCGTGG7250.032.399140
TGGGTAG7900.030.41687623
TAAGCAT8150.030.14832515
GGTAGTC7900.030.07607525
GAGTAGC8200.029.96258233
GTGTAAG8100.029.6667912
TATGCCG2550.029.64623643
TGAGTAG8350.029.42433432
CTGGGTA8400.028.92776922
Produced by FastQC (version 0.11.5)