FastQCFastQC Report
Wed 25 May 2016
SRR2074863_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074863_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1609095
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC17610.1094403997277973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG4550.039.1645939
GTAGCGT5400.037.9997635
GCGTCGT4800.037.12476738
AGCGTCG5250.037.02833637
CGTGGTA4650.035.9997742
TAGCGTC5550.035.51328736
AGTAGCG5950.034.94095634
GGTATTC4700.031.02204345
CGTTCCG350.004485429730.85694930
GTCGTGG5850.029.99981140
TCGTGGT5600.029.8926741
GGGTAGT6700.029.8207124
TATGCCG1850.029.18900743
GGTAGTC7150.028.69911825
CGACCTA507.8294403E-426.99982622
GGTGTAA7900.025.6327511
GTGTAAG7500.025.55983712
GGTATCA36300.025.5122361
GTAGTCT7950.025.13191626
CTGGGTA8200.025.02423322
Produced by FastQC (version 0.11.5)