FastQCFastQC Report
Wed 25 May 2016
SRR2074862_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074862_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences995403
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC17820.17902296858659256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTC4650.044.1281736
GTAGCGT4650.044.1281735
CGTGGTA4500.043.79914542
CGTCGTG4650.042.96690439
AGTAGCG5100.042.35211634
GCGTCGT4750.042.06233638
AGCGTCG5400.040.49920737
TATGCCG1650.039.2719643
GGTATTC4100.038.852945
GGGTAGT5450.037.65253424
TCGTGGT5250.037.02784741
CCGTCTT1700.036.52869847
CTCGTAT2050.034.24323739
GTCGTGG5850.034.1531840
GTGTAAG6200.033.0977912
GGTGTAA6400.032.9072611
GGTAGTC6350.032.73951725
TAAGCAT6850.032.32053815
CTGGGTA6650.031.67014322
TGGTGTA6300.031.28667410
Produced by FastQC (version 0.11.5)