FastQCFastQC Report
Wed 25 May 2016
SRR2074861_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074861_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1747447
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC20730.1186302073825415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG5350.037.34593239
CGTGGTA5750.033.80882342
AGCGTCG6250.033.6951637
GCGTCGT6100.033.19589238
GTAGCGT6300.032.9991835
TAGCGTC6550.031.73966836
AGTAGCG6650.031.26237934
GTCGTGG6700.031.02996640
GGTATTC5250.030.85725645
TATGCCG2650.030.5661543
TCGTGGT7000.028.15724841
GTGTAAG7500.028.08010312
GTATTCC5700.027.94667246
GGGTAGT7700.027.70060724
TACCGAA1005.2496034E-927.0016462
TAAGCAT8100.026.00084115
GAACATG20850.024.7353211
ATGCCGT3300.024.54554644
GGTGTAA8700.024.51733411
AACATGG21050.024.1154842
Produced by FastQC (version 0.11.5)