FastQCFastQC Report
Wed 25 May 2016
SRR2074860_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074860_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6231489
Sequences flagged as poor quality0
Sequence length60
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86550.027.1731511
CGAGTTA8600.024.1740729
GTATCAA142750.021.7525711
TATGCCG8400.021.53540443
CTCGTAT9750.020.21509439
ACAACGC30650.019.9978944
CAACGCA228050.018.3881155
TCTCGTA9600.017.9997438
AACAACG35750.017.9758643
AACGCAG235950.017.8868896
TCAACGC203000.017.6773764
ATCAACG204150.017.6307053
CCGTCTT9500.017.33659247
CGAGACC12350.017.05252323
TATCAAC189250.016.393552
TCGTATG13150.016.01497740
TTAGTCG5500.015.70886439
GAACATG58900.015.3574251
ATGCCGT12200.015.27027144
GAGACCG14000.015.23561724
Produced by FastQC (version 0.11.5)