FastQCFastQC Report
Wed 25 May 2016
SRR2074859_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074859_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1609015
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20000.12429964916424023No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18320.11385847863444405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3300.040.09042437
CGTCGTG3300.039.2722539
GTAGCGT3450.038.3473635
TATGCCG2350.037.91443643
TAGCGTC3650.036.24613636
CGTGGTA3550.035.74604442
GCGTCGT3900.033.23036638
CCCCCGA5250.032.9149132
AGTAGCG4050.032.6662734
TCGTGGT4100.032.26790241
CCCCGAC5550.030.6492313
GAACATG18850.029.0774751
GGTATTC3300.028.63601745
CCGTCTT2900.027.93069547
ATGCCGT3100.027.87063244
GCCCCCG6150.027.6590611
GTATTCC3500.026.99967446
GTCGTGG5000.026.99967440
CTCGTAT3300.026.18150139
TACCGAA1550.026.1295282
Produced by FastQC (version 0.11.5)