FastQCFastQC Report
Wed 25 May 2016
SRR2074858_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074858_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1573951
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG3250.044.8602754
GCGTCGT3200.040.49885638
TAGCGGA3550.040.30872334
TAGCGTC3300.040.08977536
AGCGTCG3550.037.26655637
TAGCGAT2500.035.63899232
GTAGCGT3750.035.27900335
GGTAGCG4100.034.90145532
ATTGGCG4300.034.53500421
AGCGGAT4400.033.74904635
CGTCGTG3950.032.809239
AGTAGCG4150.032.529234
GTAGCGG4400.032.5218133
CGTGGTA4200.031.4991142
GCGTGAA4550.031.4516625
TATGCCG2750.031.41729443
GAGGCGC5100.030.17753613
ATAGCGA3350.029.82005531
TCGTATG3200.029.53041540
TCTCGTA3300.029.45371238
Produced by FastQC (version 0.11.5)