FastQCFastQC Report
Wed 25 May 2016
SRR2074857_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074857_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1505687
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15730.10447058385972649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG3900.040.84625639
TAGCGTC3900.038.07575636
GTAGCGT3900.038.07575635
CGTGGTA4200.037.28580542
AGCGTCG4450.036.40337437
GCGTCGT4550.035.60448538
AGTAGCG4350.034.13688334
GGTATTC4000.032.39900245
GTCGTGG5250.030.34293440
TCGTGGT5150.029.8835741
CCCCGAC2550.028.5902043
GTATTCC4600.028.17304646
TGGTGTA5650.026.2823810
TAACGCA1650.026.1836224
TATCACG1357.2759576E-1226.0017912
GGTGTAA6050.025.88436111
GGGTAGT5900.025.62803324
GTGTAAG6050.025.43807812
CCCCCGA2900.025.1396662
ATAACGC1750.024.6874143
Produced by FastQC (version 0.11.5)