FastQCFastQC Report
Wed 25 May 2016
SRR2074856_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074856_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1398703
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15770.11274730947170343No Hit
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC14920.1066702509396205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT5000.040.49892835
CGTGGTA5050.040.0979542
CGTCGTG5050.040.0979539
TAGCGTC5000.039.41895736
GCGTCGT5150.038.27083238
AGCGTCG5300.037.69711737
AGTAGCG5700.035.5253734
TCGTGGT6000.034.6490841
TTGTACG551.0043823E-634.3639569
GGTATTC5000.032.9391345
TATGCCG2050.031.6089243
TAAGCAT6850.030.74371315
GTCGTGG6900.030.5209340
GGGTAGT6900.029.73940524
GTATTCC5600.029.40993746
TATCACG753.4556615E-728.8033542
GGTAGTC7250.028.30370925
AAGCATC7850.028.20307416
CTGAGTA7650.027.88161531
ATCTGGG7900.027.68380220
Produced by FastQC (version 0.11.5)