FastQCFastQC Report
Wed 25 May 2016
SRR2074855_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074855_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2339537
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2200.030.68142943
CGTTAGG2700.028.99963428
CGTATGC2500.025.91967241
ACGTTAG3050.023.90133727
GACGTTA3500.021.59972826
CGAGACG4150.021.46960623
ATTTACG1701.6007107E-1020.64679754
ACAACGC10800.020.5019324
TCGTATG2650.020.377140
GGTATCA45350.019.7085821
AACAACG11050.019.5493533
GTTAGGT3900.019.38436929
GAACATG22350.019.3306791
GTATCAA68250.019.0303651
CTCGTAT3300.017.99977139
ACGAGAC4950.017.99977122
CGGTTAC950.001595863717.05241640
CAACGCA100700.016.7056475
CCGTCTT3650.016.27376647
AACGCAG103850.016.2509276
Produced by FastQC (version 0.11.5)