FastQCFastQC Report
Wed 25 May 2016
SRR2074854_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074854_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743682
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA1450.033.5167428
CCGTCTT1100.031.90861547
TATGCCG1300.029.07648843
ATGCCGT1400.026.99959844
AGACGGC1800.026.99959625
TCTCGTA1158.203642E-1025.825738
TCGTATG1501.8189894E-1225.19962340
ACAACGC4450.024.2725644
CTCGTAT1502.910383E-1123.3996539
GACGGCA2000.022.94965726
GCCGTCT1554.5474735E-1122.64482546
TGCCGTC1707.2759576E-1222.23496245
ACGGCAT2100.021.85681527
CGTATGC1951.8189894E-1220.76891941
ATACAGT3400.020.6495276
CGTCTTC1701.6007107E-1020.64675148
GGTATCA14100.020.491811
CCCACGA2250.020.39969619
CACGAGA2300.019.95622421
AACAACG5150.019.9247133
Produced by FastQC (version 0.11.5)