FastQCFastQC Report
Wed 25 May 2016
SRR2074853_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074853_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1682486
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTA4450.037.61770642
TAGCGTC4600.036.39104536
CGTCGTG4500.035.99974439
GTAGCGT4700.035.61676835
AGCGTCG4950.033.8179437
GCGTCGT5000.032.3997738
AGTAGCG5250.031.88548734
TATGCCG2500.031.31977743
TCGTGGT5900.027.91505241
GTGTAAG5850.027.69211212
GGGTAGT6100.027.44242924
GTCGTGG6100.026.9998140
GGTATTC4900.026.99980745
CTGGGTA6750.024.39982622
TAAGCAT7150.024.1676615
CCGTCTT2800.024.10697247
TATCACG901.6976555E-624.0012552
TCTCGTA2700.023.99982838
GAGTAGC7100.023.95757533
ACTCGTG3500.023.91411627
Produced by FastQC (version 0.11.5)