FastQCFastQC Report
Wed 25 May 2016
SRR2074852_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074852_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3021099
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG2300.024.65217440
GGTATCA50650.024.2546881
ACAACGC12700.022.5354334
TATGCCG2750.021.60000243
AACAACG14150.020.0353363
GTATCAA77400.019.1511631
CGTATGC2550.019.05882341
CTCGTAT2850.018.9473739
TCTGTCG5650.017.6814168
CAACGCA111850.017.4528395
AACGCAG116700.016.7969156
TCAACGC100950.016.6092134
ATCAACG100800.016.5803593
TCTCGTA3750.016.56000138
GAACATG24900.015.9397591
ATCTCGT4000.015.52537
GTCTCGC4300.015.0697671
TATCAAC97100.014.9598352
ACGAGAC5650.014.81415822
TACACCG2502.6973794E-814.045
Produced by FastQC (version 0.11.5)