FastQCFastQC Report
Wed 25 May 2016
SRR2074851_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074851_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1280286
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG4050.039.9994239
TAGCGTC4400.037.43127436
CGTGGTA4200.037.28517542
GCGTCGT4500.037.1994638
GTAGCGT4400.036.81764635
TCGTGGT4300.036.4180841
TATGCCG1050.035.99947743
AGCGTCG4750.035.8100137
GTCGTGG4750.034.1047740
GGTATTC4200.033.42808545
AGTAGCG5050.033.14803334
GTGTAAG5900.029.28771412
CTCGTAT1300.029.07650239
GTATTCC5200.026.99960946
GGGTAGT6050.026.77751724
CTGGGTA6500.026.16885422
GTGGTGT7100.024.3376759
GGTGTAA7000.024.29964811
GAACATG17350.024.121631
TGGTATT6650.023.9545444
Produced by FastQC (version 0.11.5)