FastQCFastQC Report
Wed 25 May 2016
SRR2074850_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074850_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2324080
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT3350.027.40226435
GCGTCGT3500.024.68506638
CGTCGTG3550.024.33738939
TAGCGTC3900.024.23013136
AGCGTCG4200.022.4994137
GGTATCA34800.021.9577351
TATGCCG2350.021.82921243
ACAACGC9600.020.5320344
AGTAGCG4850.020.0407134
CGTGGTA4500.019.79947942
ATGCCGT2750.019.63626944
CTCGTAT3050.019.474939
AACAACG10950.018.0006893
GTATCAA50100.017.138381
TAGGACG950.001595315517.0532844
GAACATG19450.016.7975581
TCGTGGT5500.016.69047241
GTCGTGG5550.016.0536340
CAACGCA76950.015.8953465
TCTCGTA3300.015.54504738
Produced by FastQC (version 0.11.5)