FastQCFastQC Report
Wed 25 May 2016
SRR2074848_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074848_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1796253
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC23940.13327743920260676No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22720.12648552291909881No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21010.11696570583319833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG5600.040.98157539
AGCGTCG5700.040.26259637
TAGCGTC5750.039.91248736
GTAGCGT5850.039.23022535
GCGTCGT5950.039.0246738
CGTGGTA6100.037.6224342
TCGTGGT6650.036.54084441
GTCGTGG6500.036.1379640
AGTAGCG6650.035.7288334
CGAGTTA2600.031.15341229
GGTATTC5050.031.0094745
GGGTAGT7450.030.80494124
CTGGGTA7950.029.54675922
GGTAGTC8400.027.64247325
TATCACG1104.8930815E-1027.0011272
GTTTCAT8900.027.0011272
TATGCCG2950.026.54200643
TAAGCAT8550.026.21089415
GTATTCC5750.025.35616746
TGGTGTA9000.025.2003510
Produced by FastQC (version 0.11.5)