FastQCFastQC Report
Wed 25 May 2016
SRR2074847_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074847_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1332029
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16100.12086823935514918No Hit
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC15330.11508758442946812No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15320.11501251098887486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT4000.041.17500335
CGTCGTG3950.040.32911339
CGTGGTA3950.039.6455742
AGTAGCG4500.037.834
GCGTCGT4450.037.61797738
AGCGTCG4500.036.60000237
TAGCGTC4600.034.63043236
TCGTGGT4700.034.46808641
GTCGTGG5000.034.01999740
GGTCGAC801.796252E-830.3752
TCGACTC801.796252E-830.3754
TATGCCG2300.028.17391243
TAAGCAT5900.027.91525515
GGGTAGT6500.027.00000224
CCGTCTT2250.026.40000247
TGGTGTA6650.026.39097810
GTGGTGT6600.026.1818189
ATCTGGG6600.026.18181820
CTGGGTA6500.026.16923122
CTCGTAT2950.025.6271239
Produced by FastQC (version 0.11.5)