FastQCFastQC Report
Wed 25 May 2016
SRR2074845_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074845_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1460879
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC20900.1430645522319097No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18680.12786822180344848No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18060.12362420159369805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG4750.044.3362839
AGCGTCG5000.042.11946537
TAGCGTC4950.041.9994736
GTAGCGT5050.041.16779335
AGTAGCG5200.040.4994934
GCGTCGT5350.039.36398738
CGTGGTA5250.038.5709442
GTCGTGG5900.036.15208440
TCGTGGT5800.034.9133541
GGGTAGT6600.031.09051524
GGTATTC4100.030.95082545
GTGTAAG7000.029.7006412
GGTAGTC7050.029.48898725
CTGGGTA7700.028.05159422
GTTTCAT7800.027.3476772
TGGGTAG8100.026.33323
CTGAGTA8150.026.17144831
GTATTCC4550.026.1095646
AAGCATC7850.025.7958516
GGTGTAA8200.025.6834811
Produced by FastQC (version 0.11.5)