FastQCFastQC Report
Wed 25 May 2016
SRR2074844_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074844_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479853
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20050.1354864300710949No Hit
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC18650.1260260309638863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG4900.043.52837837
TAGCGTC4800.043.3102836
GTAGCGT5050.041.70083235
GCGTCGT5050.041.70083238
CGTCGTG5100.041.29199639
CGTGGTA5350.037.3438842
AGTAGCG5750.036.62420734
ATTTACG1400.034.7136854
TCGTGGT6050.034.36187441
GCGTATC502.0625843E-532.4027181
GGGTAGT6800.032.16011424
GTCGTGG6850.031.92536740
TAGCGGA2050.031.60813534
TATGCCG3650.030.32721143
TCGTATG4250.029.2220340
GGTATTC4650.029.0317545
GGTAGCG2250.028.79852132
TCTCGTA4200.028.28426438
AGCGGAT2200.028.22582435
GTAGCGG2300.028.1724733
Produced by FastQC (version 0.11.5)