FastQCFastQC Report
Wed 25 May 2016
SRR2074843_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074843_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2318083
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23270.10038467129951775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT4700.036.1908835
CGTCGTG4750.034.67310339
TAGCGTC5100.033.35238336
AGCGTCG5650.031.06142437
CGTGGTA5100.030.70536842
GCGTCGT5600.029.41022138
AGTAGCG6250.028.07952934
GGTATCA52500.027.9784851
TATGCCG3250.025.75341443
ACGTTAG4050.025.33290727
CGTTAGG4150.024.72247528
TCGTGGT6450.024.27866441
GTGTAAG7450.023.92009412
GACGTTA4500.022.79961826
CCGTCTT2850.022.73646247
GTCGTGG7600.021.67068940
GTATCAA82850.020.3691121
TGTAAGC8500.020.01229313
GTTAGGT5400.019.99966429
GAGTAGC8850.019.83017533
Produced by FastQC (version 0.11.5)