FastQCFastQC Report
Wed 25 May 2016
SRR2074841_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074841_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2158692
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3600.035.99921443
TCGTATG3850.033.661640
CGTATGC4150.032.52940841
ATTTACG3400.030.9699154
ATTGGCG3700.030.64797621
GACTCGT5100.029.6464126
ACGAGAC6000.027.89939122
TCTCGTA5050.027.26673138
ATGCCGT5000.026.99940944
TAGCGGA4350.026.06839434
ATCTCGT5150.025.68875937
GCCGTCT4450.025.4825946
AGACTCG6200.025.25751125
CTCGTAT5250.025.1994539
TTGGCGT4100.025.02384222
CTCGTGT6150.025.02384228
AGCGGAT4550.024.92253135
TGCCGTC5100.024.8818145
TCGTGTG6200.024.82203729
GGTATCA53900.024.3957731
Produced by FastQC (version 0.11.5)