FastQCFastQC Report
Wed 25 May 2016
SRR2074840_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074840_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4868938
Sequences flagged as poor quality0
Sequence length60
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88150.031.2139531
TATGCCG5950.026.31890143
TCGTATG5900.026.08432440
CTCGTAT5950.024.50380539
CGTATGC6500.023.6765441
GTATCAA151000.022.1559961
TCTCGTA7350.022.0404638
ATCTCGT7900.019.82246437
ATGCCGT7800.018.69200544
ACGAGAC11050.018.56983622
TCAACGC180050.018.2363264
ATCAACG180350.018.2359333
CAACGCA184350.018.1042775
CCCACGA11950.018.07502219
CCACGAG12150.017.9997120
TATCAAC182650.017.9323832
AACGCAG192750.017.4133596
GTATGCC10100.016.03934542
TAAACGC8100.015.6678623
GTGGTAT35750.015.6347581
Produced by FastQC (version 0.11.5)