FastQCFastQC Report
Wed 25 May 2016
SRR2074839_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074839_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2591740
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT5900.032.4912635
TAGCGTC5700.032.2102636
GCGTCGT6000.031.94973838
CGTCGTG5950.031.76444239
GGTATCA47850.030.5275751
AGCGTCG6500.030.32282637
CGTGGTA6150.030.29243542
AGTAGCG6400.029.10913834
TATGCCG3600.028.49976543
TCGTGGT7550.026.4633641
CTCGTAT4500.023.99980439
TCTCGTA4600.023.4780738
GTATCAA81200.023.0770551
ATGCCGT4450.022.44925344
GTCGTGG8700.022.03430240
GGGTAGT9000.021.59982324
GTGTAAG9550.021.20442412
GGTAGTC10150.019.68456625
GGTATTC7050.019.53175445
TCGTATG5350.019.17741240
Produced by FastQC (version 0.11.5)