FastQCFastQC Report
Wed 25 May 2016
SRR2074838_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074838_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences995593
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4050.041.99976343
CGTATGC3900.040.84592441
TCGTATG3850.040.675140
TCTCGTA3800.037.6576938
CTCGTAT3850.036.4673339
CCGTCTT3750.035.279847
ATGCCGT4750.034.67349244
GCCGTCT4000.034.4248146
ATTTACG2050.034.24371354
ATTGGCG2050.034.24371321
GTATGCC5150.033.02894242
AGCGGAT2250.032.3998235
CGTCTTC3850.032.2595648
TAGCGGA2100.032.14267734
CCACGAG6300.032.14267720
ACGAGAC6250.031.96782322
TGCCGTC4650.031.93530545
ATCTCGT4550.031.45037337
CCCACGA6300.030.42840219
CGAGACA6500.029.90752423
Produced by FastQC (version 0.11.5)