FastQCFastQC Report
Wed 25 May 2016
SRR2074837_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074837_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3113121
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC42110.1352661846423573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTC9800.038.01969536
GTAGCGT10250.037.14076635
CGTGGTA10600.035.65970642
AGCGTCG10700.035.57877337
GCGTCGT10850.035.33574338
AGTAGCG11000.035.09934634
CGTCGTG11250.033.35937539
GGTATTC10150.031.12256845
GTGTAAG13850.028.65650412
GGGTAGT14350.028.22292124
TCGTGGT14000.027.77090841
TATGCCG5200.027.51871543
CTGGGTA14750.027.45711122
CTCGTAT6100.026.99949639
GTCGTGG14500.026.62708740
GTAAGCA15300.026.29404414
GAGTAGC14500.026.2546833
GGTGTAA15400.025.59733411
TAAGCAT15800.025.29107315
GGTAGTC15750.025.19993425
Produced by FastQC (version 0.11.5)