FastQCFastQC Report
Wed 25 May 2016
SRR2074836_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074836_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2644259
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC29890.1130373386268138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTC6350.040.81828336
GTAGCGT6600.039.27213335
GCGTCGT6800.037.32296438
ATTTACG4650.036.58078854
CGTGGTA7050.036.38243542
CGTCGTG7000.036.256639
TAGCGGA4700.036.19094534
AGCGTCG7100.036.12621337
ATTGGCG4600.035.80380621
AGTAGCG7900.033.151434
GGTAGCG5100.032.82303632
GGGTAGT8850.029.89785424
TCGTGGT8750.029.00527641
CGAGTTA4600.028.76043529
TATGCCG3850.028.75281343
GTAGTCT9850.027.41075526
AGCGGAT6150.027.21910335
GTCGTGG9450.027.14244740
GGTATTC7450.026.09356545
GTAGCGG6500.025.75345833
Produced by FastQC (version 0.11.5)