FastQCFastQC Report
Wed 25 May 2016
SRR2074834_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074834_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2240824
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC26460.11808156285366454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT5250.044.22749338
CGTCGTG5500.041.72625439
CGTGGTA5700.040.73584742
AGCGTCG5900.039.35497337
GTAGCGT6050.039.2717735
TAGCGTC6100.038.5072636
TAGCGAT5350.036.84022532
TATGCCG5700.035.99912343
CTCGTAT6350.035.29047839
AGTAGCG6950.034.574734
TCGTATG7000.033.94202840
ATTGGCG3600.032.2492121
ATAGCGA6700.031.83504331
GTCGTGG7600.030.90714340
ATTTACG3550.030.42247254
TCTCGTA7100.030.42179338
TGCCGTC6750.029.9999445
CATAGCG8100.029.99926830
ATGCCGT6850.029.9561444
GGGTAGT8150.029.48394424
Produced by FastQC (version 0.11.5)