FastQCFastQC Report
Wed 25 May 2016
SRR2074833_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074833_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3301211
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG4800.038.2494339
GTAGCGT5550.033.56706635
AGCGTCG5850.031.8456837
CGTGGTA5700.030.31533642
TAGCGTC6150.029.85321236
AGTAGCG6300.029.57098834
GCGTCGT6400.028.26520738
CGAGACT6500.024.0919523
GGTATCA63950.023.307481
TCGTGGT7950.022.4147641
GGTATTC6800.021.04380645
GAACATG28950.020.9860521
GTATCAA100900.020.713171
GGGTAGT8750.020.36541224
GTCGTGG9450.020.28541240
AACATGG30250.019.727122
ATCGCGT857.5670535E-419.0585437
GGTGTAA9750.018.83077411
CACGAGA9150.017.99973321
ACGAGAC9300.017.99973322
Produced by FastQC (version 0.11.5)