FastQCFastQC Report
Wed 25 May 2016
SRR2074832_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074832_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2161037
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT4500.038.3993935
GCGTCGT4750.037.51519438
TAGCGTC4650.037.160736
CGTCGTG4750.035.80995639
CGTGGTA5000.035.63943542
AGTAGCG5800.031.18915734
AGCGTCG5650.031.06145737
TATGCCG4250.029.85834943
ATTTACG2450.029.7546354
ATTGGCG2900.028.86160921
TCGTGGT6100.028.32742141
GTCGTGG6300.027.85670340
TAGCGGA3600.026.99956934
GCGTGAA3250.026.58419225
CTCGTAT4900.026.44855939
TCGTATG5150.026.21317940
GGTAGCG3650.025.88999732
GGGTAGT7450.025.36872724
CCGTCTT4800.025.31209847
GGTATCA40650.024.6439571
Produced by FastQC (version 0.11.5)