FastQCFastQC Report
Wed 25 May 2016
SRR2074831_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074831_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2602987
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC30840.11847927016154902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG7850.041.96138439
GTAGCGT8400.039.53533635
AGTAGCG8650.038.0805634
AGCGTCG8800.037.73827437
GCGTCGT8750.037.02821438
CGTGGTA8700.036.9306842
TAGCGTC8900.036.7075136
ACGTTAG6100.034.08164227
GTCGTGG10150.032.98490540
GTGTAAG10750.032.4003112
TCGTGGT10050.032.238541
GGGTAGT10800.031.250324
TCTCGTA5150.030.93174238
CTCGTAT5300.030.56574439
GACGTTA6900.030.52144826
TATGCCG5400.030.49970643
CGTTAGG7000.029.69971528
GGTATTC9450.028.57115645
TAAGCAT12700.027.8506615
GGTGTAA12700.027.21233611
Produced by FastQC (version 0.11.5)