FastQCFastQC Report
Wed 25 May 2016
SRR2074830_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074830_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660396
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6820.10327137051102672No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA2000.036.44999728
TTAGTCG451.0023337E-536.00000439
TATGCCG1650.034.36363643
TAGTCGG502.0620482E-532.39999840
CTCGTAT1850.030.64864739
TCTCGTA1700.030.17647238
TCGTATG2250.028.840
GACGGCA2450.028.6530626
GCATAGG2700.028.00000230
TGCCGTC1950.027.69230845
CCGTCTT1800.027.00000247
GTAGCGT905.6315912E-827.00000235
TTCGCTC400.0086077527.045
TGCCGCG400.0086077527.05
GCGTCGT1005.2405085E-926.99999838
AGACGGC2650.026.49056625
GAACATG5350.025.7383171
AGCGTCG1058.811185E-925.71428537
GGCATAG2950.025.6271229
CGTGGTA959.509313E-825.57894742
Produced by FastQC (version 0.11.5)