FastQCFastQC Report
Wed 25 May 2016
SRR2074829_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074829_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2145453
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC35740.16658486576028467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT8300.043.2644135
CGTCGTG8350.042.68198839
CGTGGTA8550.041.99936342
TAGCGTC8800.041.4198336
AGTAGCG9050.040.57397534
GCGTCGT8950.040.42397338
AGCGTCG9150.039.83546437
GGTATTC7700.039.27213745
TCGTGGT10100.036.35588541
GTCGTGG10100.036.08856240
GTGTAAG10800.034.00107212
GTATTCC9150.033.63883646
TATGCCG4450.032.7635543
ACTCGTG5750.032.3995127
GGGTAGT11350.032.35193324
GTAAGCA12100.031.24065214
TAAGCAT12100.031.24065215
GAGTAGC11900.031.08356333
CTGGGTA12250.030.85667622
GGTGTAA12350.030.17028811
Produced by FastQC (version 0.11.5)