FastQCFastQC Report
Wed 25 May 2016
SRR2074828_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074828_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2152674
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC48120.22353593716466125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG10900.044.09062639
GTAGCGT11400.042.63049335
CGTGGTA11300.042.05203242
AGCGTCG11750.042.0537
TAGCGTC11800.041.6430136
AGTAGCG11950.041.12029634
GCGTCGT11950.040.4424938
TCGTGGT12600.037.92761241
GGTATTC10750.037.925545
GGGTAGT13300.036.7442824
GTGTAAG13600.035.73604612
GTCGTGG13600.035.53586640
TAAGCAT14300.034.55317715
CTGGGTA14150.033.96616722
GTATTCC12300.033.80480646
TATGCCG3300.033.54460543
GGTAGTC15100.032.54297625
TGGTGTA15400.031.90976110
ATCTGGG15800.031.61605820
TGAGTAG15650.031.39856332
Produced by FastQC (version 0.11.5)