FastQCFastQC Report
Wed 25 May 2016
SRR2074827_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074827_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1855469
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC25120.13538356070621496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG5700.041.68329639
TAGCGTC5850.041.07602336
GTAGCGT5800.040.9646235
AGCGTCG6100.039.83519437
CGTGGTA6050.039.27186642
GCGTCGT6150.038.63329338
ATTTACG3450.037.56540754
AGCGGAT3950.035.5435235
AGTAGCG6950.035.3517434
ATTGGCG3900.034.61462421
TAGCGGA4000.034.42424434
TCGTGGT7050.033.31841741
GGGTAGT7300.032.91708824
TATGCCG3100.032.225143
GGTATTC6300.030.42872245
GGTAGCG4700.029.87168532
TGGGTAG8200.029.30423523
TAAGCAT8500.028.90524915
TCGTATG3950.028.7082340
GTCGTGG8550.027.78886440
Produced by FastQC (version 0.11.5)